Characteristics of Female Genital Opportunistic Infection Who Infected with Human Immunodeficiency Virus / 中华医院感染学杂志

OBJECTIVE To investigate the characteristics of female genital opportunistic infection patients who infected with human immunodeficiency virus(HIV) and to provide reference for early diagnosis and treatment of HIV infection.METHODS The clinical data of 116 patients with HIV,including female genital symptoms and general condition collected from Jan 2007 to Dec 2008 were analyzed retrospectvely.These patients were all from the Central African Republic.RESULTS Among these 116 patients with HIV,89 patients(76.72%)had infective changes in the female genitals,and 68 patients(58.62%) had two to four diseases simultaneously.Among these infections,the most infection was chronic infection in the basin behaved chronic ache in the basin and infertility,then were vulvovaginal candidiasis,acute pelvic inflammatory disease,and trichomonal vaginitis and cervicitis.The CD4+ cell counts of 67 patients decreased,and the CD8+ cell counts of 31 patients decreased,of which 35 patients were accompanied with chronic dissipative symptoms among these patients.CONCLUSIONS HIV can descend human immunity,the patients can suffer with female genital oppprtunistic infection easily and should be paid more attention to in the eary stage.

Characterization of The Essential Gene Components for Conjugal Transfer of Streptomyces lividans Linear Plasmid SLP2 / 生物化学与生物物理进展

Commonly, the interbacterial transfer of circular plasmids is initiated by nicking at an internal sequence, oriT, followed by transferring one strand as single-stranded DNA through a type Ⅳ secretion channel on cell membrane. In contrast, Streptomyces conjugative linear plasmids, containing a free 3'-end but a protein-capped 5'-end, can potentially undergo cell-to-cell transfer by transfer of non-nicked DNA. It was reported that circular derivatives of the Streptomyces lividans linear plasmid SLP2, as well as the parental linear plasmid itself can transfer efficiently. And the genetic requirements for such transfer was described. Efficient transfer of plasmid requires six co-transcribed SLP2 genes, encoding a Tra-like DNA translocase, cell wall hydrolase, two cell membrane proteins that interact with an ATP binding protein, and a protein of unknown function. Reduced transfer efficiency of plasmid from SalⅠ R-/M-to Sal Ⅰ R/M hosts argues that transfer of both the circular and linear forms of the plasmid involves double-stranded DNA. These results suggest that conjugal transfer occurs by a similar mechanism for SLP2-derived linear and circular plasmids, and cellular membrane/wall functions in the transfer process.